α(1-2,3,6)-Mannosidase (jack bean)

Popis produktu: Agilent Exoglycosidases (formerly ProZyme) are glycoside hydrolase enzymes that cleave the glycosidic bonds at the terminal residues. These enzymes are useful in the study of isolated glycans, glycolipids, glycoproteins. Linkage-specific exoglycosidases may also be used for sequencing of oligosaccharides. When used in combination, exoglycosidases enable flexible analytical tools for glycan analysis.

Má expirační dobu: Ne
Standardní složky SF: -
%  uhlíku: -
Koncentrace: 150 U/mL
Uzavřená: -
Bio Aplikace: -
Zdroj enzymu: Jack bean.
Enzymová aktivita: -
Specifita enzymu: The enzyme has broad specificity, cleaving a(1-2,3,6)-linked mannose, although some kinetic preference has been observed (a1-2, 6 3). The enzyme will not cleave a single a(1-6) linked mannose residue from core ß-mannose, but will, however, remove a single a1-3 linked mannose from the core ß-mannose. By using enzyme concentrations of around 50 U/ml and extended incubation times (up to 18 hours) at 37°C, complete removal of all nonreducing terminal a-linked mannose residues may be achieved. To expedite glycan sequencing studies, the sluggish activity of GKX-5010 jack bean a mannosidase toward a(1-6)-linked mannose residues can be overcome by using the enzyme in combination with the a mannosidase from Xanthomonas mannihotis that rapidly cleaves a(1-6) linkages. The X. mannihotis a-mannosidase may inhibit the action of other mannosidases if a branched (a1-6) mannose is present in the substrate, so is typically added after incubation of the substrate with jack bean a-mannosidase (GKX-5010).
Definice enzymové jednotky: One unit is defined as the amount of enzyme that will hydrolyze 1 µmole of pNP-?-mannopyranoside per minute at pH 4.5 and 37°C.
Analýza Glykanů Aplikace: -
Předkolona: -
Vnitřní průměr: -
Požadavky pro použití kitu: -
Typ kolony: -
Maximální teplota kolony: -
Stacionární fáze (SF): -
Typ chromatografie: -
Délka kolony: -
Dolní mez molekulové hmotnosti: -
Horní mez molekulové hmotnosti: -
Molekulová hmotnost: ~190 kD
Velikost částic:
Typ částic:
Optimální pH: 4.5
Rozsah pH: -
Velikost pórů: -
Tlak na koloně: -
Rozsah obsahu proteinů:
Separační mód: -
USP kód -
UNSPSC kód: 12352204

Více informací na stránkách výrobce: www.chem.agilent.com/search/