AdvanceBio PNGase A (almond), 2mU

Popis produktu: Agilent Endoglycosidases (formerly ProZyme) release oligosaccharides from glycoproteins and some other substrates. These enzymes are useful for deglycosylating glycoproteins prior to analysis, or for releasing N-glycans for labeling and analysis. Useful in N-glycan analysis, Agilent offers PNGase F, an asparagine amidase that releases intact N-glycans. PNGase F cleaves between the innermost GlcNac of the N-glycan and the asparagine (Asn) residue of the peptide to which the N-glycan is linked

Má expirační dobu: Ano
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Zdroj enzymu: Almond. Enzyme also knows as Peptide N-Glycosidase A, Glycopeptidase from almonds, Almond glycoamidase, Peptide-N(4)-(N-acetyl-? glucosaminyl) asparagine amidase.
Enzymová aktivita: 5 units/mg
Specifita enzymu: PNGase A from almonds cleaves N-glycan chains linked to Asparagine from glycopeptides. The enzyme hydrolyzes an N(4)-(acetyl-ß D-glucosaminyl) asparagine residue in which the glucosamine residue may be further glycosylated, to yield a (substituted) N-acetyl-ß D-glucosaminylamine and a peptide containing an aspartate residue. The enzyme cleaves N-glycans with or without a(1,3)-linked core fucose residues present in insect and plant glycoproteins. This differs from PNGase F, which cannot remove N-glycans with a(1,3)-linked core fucose. PNGase A requires glycopeptides as substrates. Glycopeptides can be obtained by digesting the glycoprotein with trypsin [1,2] or pepsin. Does not act on (GlcNAc)-Asn, because it requires the presence of more than two amino acid residues in the substrate.
Definice enzymové jednotky: One unit is defined as the amount of enzyme required to hydrolyze 1 µmol ovalbumin glycopeptide per min at pH 5.0 and 37°C.
Analýza Glykanů Aplikace: -
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Molekulová hmotnost: 54.2 and 21.2 kDa
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Optimální pH: 5
Rozsah pH: 4-6
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UNSPSC kód: 12352204

Více informací na stránkách výrobce: www.chem.agilent.com/search/