AdvanceBio β-N-Acetylhexosaminidase

Popis produktu: Agilent Exoglycosidases (formerly ProZyme) are glycoside hydrolase enzymes that cleave the glycosidic bonds at the terminal residues. These enzymes are useful in the study of isolated glycans, glycolipids, glycoproteins. Linkage-specific exoglycosidases may also be used for sequencing of oligosaccharides. When used in combination, exoglycosidases enable flexible analytical tools for glycan analysis.

Má expirační dobu: Ano
Standardní složky SF: -
%  uhlíku: -
Koncentrace: 40 U/mL
Uzavřená: -
Bio Aplikace: Due to the complex nature of the specificity of this enzyme, it has been used extensively for the analysis of the positional isomerism of GlcNAc in glycans. It is complementary to the ß-N-acetylhexosaminidase from jack bean (GKX-5003).
Zdroj enzymu: Recombinant gene from Streptococcus pneumoniae, expressed in E. coli.
Enzymová aktivita: -
Specifita enzymu: This enzyme releases nonreducing terminal ß(1-2,3,4, and 6)-linked N-acetylglucosamine from complex carbohydrates. When incubated with oligosaccharides at low concentrations (less than 50 mU/ml) the enzyme can differentiate between GlcNAcß1-2Man, GlcNAcß1-4Man and GlcNAcß1-6Man linkages. Under such conditions, the enzyme cleaves only ß1-2 linked GlcNAc, with two provisos. First, ß1-2 GlcNAc is not hydrolyzed if the mannose to which it is substituted has a substitution at C-6. Thus, the enzyme is useful for the analysis of triantennary oligosaccharides. Second, if the ß-linked mannose of the conserved pentasaccharide core is substituted with a "bisecting" GlcNAc then only the ß1-2 linked GlcNAc linked to mannose on the a1-3 arm is cleaved. At higher concentrations of the enzyme, ß(1-4) and ß(1-6)-linked GlcNAc may also be hydrolyzed. The reported activity against O-linked oligosaccharides suggests that GlcNAc ß1-3Gal and GlcNAc ß1-6Gal are digested1. Although the specificity towards oligosaccharides containing GalNAc is not known, the enzyme, in common with other glucosaminidases, also hydrolyses pNP-ß-GalNAc at pH 6.0 and 37C. The activity towards this substrate is ~7 fold less than towards pNP-ßGlcNAc.
Definice enzymové jednotky: One unit is defined as the amount of enzyme required to catalyze the release of 1 µmole of p-nitrophenol from p-nitrophenol-N-acetyl-ß-D-glucosaminide per minute at 37°C, pH 5.0
Analýza Glykanů Aplikace: -
Předkolona: -
Vnitřní průměr: -
Požadavky pro použití kitu: -
Typ kolony: -
Maximální teplota kolony: -
Stacionární fáze (SF): -
Typ chromatografie: -
Délka kolony: -
Dolní mez molekulové hmotnosti: -
Horní mez molekulové hmotnosti: -
Molekulová hmotnost: -
Velikost částic:
Typ částic:
Optimální pH: 5
Rozsah pH: -
Velikost pórů: -
Tlak na koloně: -
Rozsah obsahu proteinů:
Separační mód: -
USP kód -
UNSPSC kód: 12352204

Více informací na stránkách výrobce: www.chem.agilent.com/search/