AdvanceBio Sialidase S, His-tagged

Popis produktu: Agilent Exoglycosidases (formerly ProZyme) are glycoside hydrolase enzymes that cleave the glycosidic bonds at the terminal residues. These enzymes are useful in the study of isolated glycans, glycolipids, glycoproteins. Linkage-specific exoglycosidases may also be used for sequencing of oligosaccharides. When used in combination, exoglycosidases enable flexible analytical tools for glycan analysis.

Má expirační dobu: Ano
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Zdroj enzymu: AdvanceBio Sialidase S. Recombinant from Streptococcus pneumoniae, expressed in E. coli. 6xHis-tagged. Sialidases are also known as neuraminidases.
Enzymová aktivita: -
Specifita enzymu: This enzyme preferentially releases a(2-3)-linked N-acetylneuraminic acid (Neu5Ac, NANA) from complex carbohydrates. Sialidase S is also active against a(2-3)-linked N-glycolyneuraminic acid [1] (Neu5Gc, NGNA), although similarly to other sialidases [2] the activity is lower toward Neu5Gc than Neu5Ac. Although the a(2-3)-linkage is preferred, Sialidase S (also known as NanB) is also capable of cleaving a(2-6)-linked sialic acid [3], particularly at higher concentrations. Adding an excess of this enzyme can lead to some cleavage of a(2-6)-linked sialic acid. Using GK80021 as directed in the certificate of analysis reduces the likelihood of these effects. Users may wish to optimize the enzyme concentration needed for specific proteins. May be used for exoglycosidase sequencing with Sialidase A (GK80040), which has a broader specificity, releasing a(2-3,6,8,9)-linked sialic acids. Sialidases are also known as neuraminidases.
Definice enzymové jednotky: One unit is defined as the amount of enzyme required to catalyze the release of 1 µmole of p-nitrophenol from p-nitrophenyl-?-D-N-acetylneuraminic acid per minute at 37° C, pH 5.5
Analýza Glykanů Aplikace: -
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Molekulová hmotnost: ~75 kD
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USP kód -
UNSPSC kód: 12352204

Více informací na stránkách výrobce: www.chem.agilent.com/search/