AdvanceBio N-Glycanase (PNGase F), >2.5

Popis produktu: Agilent Endoglycosidases (formerly ProZyme) release oligosaccharides from glycoproteins and some other substrates. These enzymes are useful for deglycosylating glycoproteins prior to analysis, or for releasing N-glycans for labeling and analysis. Useful in N-glycan analysis, Agilent offers PNGase F, an asparagine amidase that releases intact N-glycans. PNGase F cleaves between the innermost GlcNac of the N-glycan and the asparagine (Asn) residue of the peptide to which the N-glycan is linked

Má expirační dobu: Ano
Standardní složky SF: -
%  uhlíku: -
Koncentrace: 2.5 U/mL
Uzavřená: -
Bio Aplikace: Release of intact N-linked glycans from glycopeptides and glycoproteins;Structure-function studies of N-glycosylated glycoproteins;Preparation of deglycosylated proteins for molecular weight estimation or crystallography studies
Zdroj enzymu: Recombinant gene from Elizabethkingia meningoseptica, expressed in E. coli. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum. Enzyme is also known as peptide-N-glycosidase F, peptide-N4-(N-acetyl-?-glucosaminyl)asparagine amidase.
Enzymová aktivita: ?10 units/mg
Specifita enzymu: Cleaves all N-linked complex, hybrid or high mannose oligosaccharides, unless a(1-3) core fucosylated, as in plant glycans and some insect glycans. Asparagine must be peptide bonded at both termini. Phosphate, sulfate and sialic acid groups attached to the oligosaccharide do not affect cleavage. Endo F free.
Definice enzymové jednotky: One unit is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 µmole of denatured ribonuclease B per minute at 37°C, pH 7.5.
Analýza Glykanů Aplikace: -
Předkolona: -
Vnitřní průměr: -
Požadavky pro použití kitu: -
Typ kolony: -
Maximální teplota kolony: -
Stacionární fáze (SF): -
Typ chromatografie: -
Délka kolony: -
Dolní mez molekulové hmotnosti: -
Horní mez molekulové hmotnosti: -
Molekulová hmotnost: ~35,000 daltons
Velikost částic:
Typ částic:
Optimální pH: 8.6
Rozsah pH: 7.5-9.5
Velikost pórů: -
Tlak na koloně: -
Rozsah obsahu proteinů:
Separační mód: -
USP kód -
UNSPSC kód: 12352204

Více informací na stránkách výrobce: www.chem.agilent.com/search/