AdvanceBio N-Glycanase (PNGase F), >2.5

Popis produktu: Agilent Endoglycosidases (formerly ProZyme) release oligosaccharides from glycoproteins and some other substrates. These enzymes are useful for deglycosylating glycoproteins prior to analysis, or for releasing N-glycans for labeling and analysis. Useful in N-glycan analysis, Agilent offers PNGase F, an asparagine amidase that releases intact N-glycans. PNGase F cleaves between the innermost GlcNac of the N-glycan and the asparagine (Asn) residue of the peptide to which the N-glycan is linked

Má expirační dobu: Ne
Standardní složky SF: -
%  uhlíku: -
Koncentrace: ?2.5 U/mL
Uzavřená: -
Bio Aplikace: Release of intact N-linked glycans from glycopeptides and glycoproteins;Structure-function studies of N-glycosylated glycoproteins;Preparation of deglycosylated proteins for molecular weight estimation or crystallography studies
Zdroj enzymu: Recombinant gene from Elizabethkingia meningoseptica, expressed in E. coli. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum. The enzyme is also known as PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-?-glucosaminyl)asparagine amidase.
Enzymová aktivita: ?10 units/mg
Specifita enzymu: Cleaves all N-linked complex, hybrid or high mannose oligosaccharides, unless ?(1-3) core fucosylated, as in plant glycans and some insect glycans. Asparagine must be peptide-bonded at both termini. Phosphate, sulfate, and sialic acid groups attached to the oligosaccharide do not affect cleavage. This product is Endo F free.
Definice enzymové jednotky: One unit is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 ?mole of denatured ribonuclease B per minute at 37°C, pH 7.5.
Analýza Glykanů Aplikace: -
Předkolona: -
Vnitřní průměr: -
Požadavky pro použití kitu: -
Typ kolony: -
Maximální teplota kolony: -
Stacionární fáze (SF): -
Typ chromatografie: -
Délka kolony: -
Dolní mez molekulové hmotnosti: -
Horní mez molekulové hmotnosti: -
Molekulová hmotnost: ~35,000 daltons
Velikost částic:
Typ částic:
Optimální pH: 8.6
Rozsah pH: 7.5-9.5
Velikost pórů: -
Tlak na koloně: -
Rozsah obsahu proteinů:
Separační mód: -
USP kód -
UNSPSC kód: 12352204

Více informací na stránkách výrobce: www.chem.agilent.com/search/