PNGase F (native, Elizabethkingia mening

Popis produktu: Agilent Endoglycosidases (formerly ProZyme) release oligosaccharides from glycoproteins and some other substrates. These enzymes are useful for deglycosylating glycoproteins prior to analysis, or for releasing N-glycans for labeling and analysis. Useful in N-glycan analysis, Agilent offers PNGase F, an asparagine amidase that releases intact N-glycans. PNGase F cleaves between the innermost GlcNac of the N-glycan and the asparagine (Asn) residue of the peptide to which the N-glycan is linked

Má expirační dobu: Ano
Standardní složky SF: -
%  uhlíku: -
Koncentrace: ?2 U/mL
Uzavřená: -
Bio Aplikace: PNGaseF is used to release intact N-linked glycans from glycopeptides and glycoproteins;structure-function studies of N-glycosylated glycoproteins;preparation of deglycosylated proteins for molecular weight estimation or crystallography studies
Zdroj enzymu: Native, purified from Elizabethkingia meningoseptica. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum
Enzymová aktivita: -
Specifita enzymu: PNGase F releases intact N-linked oligosaccharides from glycoproteins and glycopeptides. The enzyme will not cleave oligosaccharides from a single asparagine residue and cleavage does not occur if there is core ?1-3 linked fucose as commonly encountered in plant glycoproteins Phosphate, sulfate and sialic acid groups attached to the oligosaccharide do not affect cleavage.
Definice enzymové jednotky: One unit of N-Glycanase is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 ?mole of denatured ribonuclease B per minute at pH 7.5 and 37°C
Analýza Glykanů Aplikace: Structural analysis of O-glycan
Předkolona: -
Vnitřní průměr: -
Požadavky pro použití kitu: -
Typ kolony: -
Maximální teplota kolony: -
Stacionární fáze (SF): -
Typ chromatografie: -
Délka kolony: -
Dolní mez molekulové hmotnosti: -
Horní mez molekulové hmotnosti: -
Molekulová hmotnost: -
Velikost částic:
Typ částic:
Optimální pH: 7.5
Rozsah pH: -
Velikost pórů: -
Tlak na koloně: -
Rozsah obsahu proteinů:
Separační mód: -
USP kód -
UNSPSC kód: 12352204

Více informací na stránkách výrobce: www.chem.agilent.com/search/