AdvanceBio Sialidase A, 1 U

Popis produktu: Agilent Exoglycosidases (formerly ProZyme) are glycoside hydrolase enzymes that cleave the glycosidic bonds at the terminal residues. These enzymes are useful in the study of isolated glycans, glycolipids, glycoproteins. Linkage-specific exoglycosidases may also be used for sequencing of oligosaccharides. When used in combination, exoglycosidases enable flexible analytical tools for glycan analysis.

Má expirační dobu: Ano
Standardní složky SF: -
%  uhlíku: -
Koncentrace: 5 U/mL
Uzavřená: -
Bio Aplikace: -
Zdroj enzymu: Recombinant gene from Arthrobacter ureafaciens, expressed in E. coli. Sialidases are also known as neuraminidases.
Enzymová aktivita: 40 units/mg
Specifita enzymu: The enzyme releases a(2,3)-, a(2,6)-, a(2,8)-, and a(2,9)-linked N-acetylneuraminic acid from complex carbohydrates. The initial rate of hydrolysis of a(2,6) linkages is reported to be approximately twice that of a(2,3)-linked sialic acid however, in practice, this kinetic selectivity is of little consequence during extended incubations. Effective digestion of glycolipid substrates is facilitated by addition of a detergent, such as sodium taurodeoxycholate to the incubation. Sialidase A is capable of releasing N-glycolylneuraminic acid (Neu5Gc, NGNA) in addition to N-acetylneuraminic acid (Neu5Ac, NANA) [1], although similarly to other sialidases [2] the activity is lower toward Neu5Gc than Neu5Ac. The enzyme can also be used to remove sialic acid from gangliosides [3], glycosphingolipids (ceramide and oligosaccharide) with sialic acid. May be used for exoglycosidase sequencing with Sialidase S (GK80021), which is specific for a(2,3)-linked sialic acids. Sialidases are also known as neuraminidase.
Definice enzymové jednotky: One unit is defined as the amount of enzyme required to catalyze the release of 1 µmole of p-nitrophenol from p-nitrophenyl-?-D-N-acetylneuraminic acid per minute at 37° C, pH 5.5.
Analýza Glykanů Aplikace: -
Předkolona: -
Vnitřní průměr: -
Požadavky pro použití kitu: -
Typ kolony: -
Maximální teplota kolony: -
Stacionární fáze (SF): -
Typ chromatografie: -
Délka kolony: -
Dolní mez molekulové hmotnosti: -
Horní mez molekulové hmotnosti: -
Molekulová hmotnost: ~88 kD
Velikost částic:
Typ částic:
Optimální pH: 6
Rozsah pH: -
Velikost pórů: -
Tlak na koloně: -
Rozsah obsahu proteinů:
Separační mód: -
USP kód -
UNSPSC kód: 12352204

Více informací na stránkách výrobce: www.chem.agilent.com/search/